Journal: Briefings in Bioinformatics

Article Title: Subset binding enables detection of multimodal patient subgroup patterns and drug target discovery in idiopathic pulmonary fibrosis

doi: 10.1093/bib/bbag153

Figure Lengend Snippet: Ponatinib treatment attenuated TGF-β-induced expression of EMT genes/markers. Cells were treated with ponatinib or SB431542. Subsequently, rTGF-β was added to the cells and cultured for 48 h. Furthermore, cells were subjected to RT-qPCR for the specified transcripts of a ) E-cadherin, b ) α-SMA, c ) fibronectin, and d ) Snail. During the progression of EMT, the expression of E-cadherin and α-SMA showed little change in our EMT model, while the expression of fibronectin and Snail was upregulated. Treatment with the positive control SB431542 (upper panel) attenuated the upregulation of fibronectin and Snail in a dose-dependent manner. A similar dose-dependent pattern was observed upon treatment with ponatinib (lower panel), suggesting that ponatinib inhibits TGF-β-induced expression of EMT at least partially, aligning with the network analysis. Relative expression levels are presented as the means.

Article Snippet: Cell lysis and RT were performed using the SuperPrep® II Cell Lysis & RT Kit for qPCR (Toyobo Co., Ltd.), and the expression of the aforementioned EMT marker genes was measured.

Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Positive Control

Journal: iScience

Article Title: Horizontal mtDNA transfer between cells is common during mouse development

doi: 10.1016/j.isci.2022.103901

Figure Lengend Snippet:

Article Snippet: Purogen DNA purification Kit , Qiagen , # 158908; # 158912; # 158914.

Techniques: Recombinant, DNA Extraction, DNA Purification, Sequencing, Mutagenesis, Software